Chromatography Calculations
Retention Factor
k = (tR - t0) / t0
tR: Holding time, t0: Dead time
Resolution
Rs = 2(tR2 - tR1) / (w1 + w2)
Resolution between two peaks
The Selectivity Factor
α = k2 / k1
Selectivity between two components
Theoretical Plate Count
N = 16(tR / w)²
Column efficiency

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